Wine Cap Liquid Culture Syringe

Wine Cap Liquid Culture Syringe

Original price was: $50.00.Current price is: $15.00.

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Product Short Description

10-milliliter sterile medical syringe containing live Stropharia rugosoannulata (Wine Cap Mushroom) Liquid Culture Syringe. Each order includes one sterile, unopened dispensing needle.

 

 

Description

Wine Cap Mushrooms

Wine Cap mushrooms, scientifically known as Stropharia rugosoannulata, are a robust and adaptable species, renowned for their rapid growth on various types of woody debris. One of their most notable features is their resilience to a wide range of environmental conditions, including an ability to tolerate some levels of sunlight. This characteristic makes them particularly well-suited for integration into vegetable gardens. Their versatility in different environments, coupled with their vigorous growth, positions Wine Caps as an excellent choice for mushroom cultivators and garden enthusiasts seeking to incorporate fungi into their ecosystems.

Morphologically, Wine Cap mushrooms bear a resemblance to portobellos, which aids in their identification. They develop a classic cap-and-stem structure, with their caps notably exhibiting a deep burgundy color. This hue can vary from bright red to purplish shades, depending on sun exposure, and may fade to medium brown. The caps often display fluffy white tufts, particularly near the margins and more prominently in younger mushrooms. The gills of Wine Caps are purple-gray, turning dark purple-brown when filled with spores of the same color. Their flesh and stem are cream to white, featuring a distinctive ring covering the gills in younger stages. As they mature, this ring, which is radially split and not fibrous like Amanitas or cobwebby like Cortinarius, travels down the stem, often breaking off in chunks or resembling a cogwheel. This unique feature further distinguishes Wine Caps in the diverse world of mycology.

Overview of The Wine Cap Liquid Culture Syringe

Wine Cap Liquid Culture Syringe or LC, is perfect for your home mushroom research. This easy-to-use syringe is pre-filled with a living mycelium suspended in a nutrient broth of the famous Wine Cap mushroom strain, so you can begin researching the mushrooms immediately! In addition, our liquid culture syringes are made from high-end isolations that will speed your research efforts up to speed much faster than our spores!

Product Volume: 10 Milliliters

Ingredients: Thick Spores brand mushroom liquid culture syringes are made with 100% natural ingredients and are non-toxic.

Culture media: Extra Light Malt Extract or Potato Dextrose for maximum shelf life and protection from senescence.

Usage

How to use a mushroom liquid culture syringe:

  1. Prepare the work area: Before you begin, ensure your workspace is clean and sanitized. You will need a clean surface to rest the syringe on and safe disposal for any used materials.
  2. Handle the syringe with care to avoid any accidental spills or contamination.
  3. Prepare the solution: Open the mushroom liquid culture syringe and make sure that the aqueous solution is clear, other than the mycelium. If the aqueous solution is cloudy, discard it and start with a new one.
  4. Prepare a microscopy slide or sanitize the area to be penetrated by the dispensing needle
  5. Dispense a small amount of the solution onto a microscopy slide, or inject 1 milliliter into the container you will use through a self-healing injection port.

Tips and Tricks for successful usage

  • Always be as clean as possible.
  • Wear clean clothing.
  • Practice good oral hygiene.
  • Wear new gloves every time.
  • Wipe all surfaces, including gloves, with disinfectant.
  • Do not introduce un-sanitized items into a sanitized work area.

Advantages of a liquid culture syringe over a spore syringe

A liquid culture syringe has a significant advantage over a spore syringe in terms of the time you do not have to wait for germination. A liquid culture syringe will save you weeks to months in your endeavor. Another advantage is that liquid cultures can be advanced genetic isolates that can outperform spores. Thick Spores sells only genetically isolated LC.

Shelf Life & Storage

Liquid Culture syringes have a shelf life of approximately three months before the nutrient broth becomes depleted of nutrients. Therefore, you must either use the LC before three months or transfer the mycelium in the syringe to a new and sterile medium with fresh nutrients.

 

 

Thick Spores 30-Day Product Guarantee:

At Thick Spores, we are committed to delivering high-quality products. If you encounter any issues, please refer to the guidelines below for our warranty terms.

Please Note: Our products are not intended for human consumption.

Spores and Liquid Culture Warranty Terms:

1. Damages During Shipping:
If your order is damaged due to carrier mishandling, please contact us within 24 hours of delivery with photos of all packaging and damaged items.

  • We will dispatch a replacement order free of charge on the next business day, provided you follow the above instructions.
  • We cannot offer refunds for packages damaged during transit. However, we will file a claim with the carrier's insurance and send a replacement order.

 

2. Contamination:

  • Our production utilizes a professional-grade laminar flow HEPA flowhood with a 12" thick filter, filtering to 0.02 microns. Therefore, contamination is highly unlikely to originate from our end.
  • To claim contamination, test the spores or liquid culture on agar and provide photographs showing our company name and the date. Also include pictures and descriptions of your equipment, again with our company name in the picture for verification puposes
  • We do not cover contaminated grain or third-party grow kits. Our warranty does not extend to customer errors in substrate preparation or third-party vendor issues.

3. Viability:

  • All batches are tested for viability prior to dispatch.
  • Non-viability is often due to mishandling post-delivery. Please bring your orders inside within 10 minutes of delivery.
  • If you cannot be home for delivery, arrange for an indoor PO BOX or similar for safe delivery.
  • Do not store your items in the refrigerator. It can become too cold. Room temperature is optimal.
  • Thick Spores is not liable for customer negligence.
  • For extreme climates, consider our next day air shipping option.
  • If carrier mishandling (e.g., delays or lost orders) results in viability issues, we may replace the order at our discretion and a fee may be required.

Before Leaving a Review:

  • If you experience any issues, please contact us before leaving a review.
  • Leaving a review that is less than 5 stars without following our policy or contacting us will result in the refusal of future purchases.
  • We strive for your satisfaction and will do our best to resolve any issues.

Thank you for choosing Thick Spores! ??

Frequently Asked Questions

What is a mushroom liquid culture?

A mushroom liquid culture is living mushroom mycelium suspended within a nourishing nutrient broth, typically stored inside a medical-grade syringe or mason jar. 

The nutrient broth is yellow or golden due to the ingredients that it is made with. Once the nutrient broth is pressure-cooked, the nutrients react to the heat inside the pressure cooker as the nutrient broth is sterilized. The reaction is mainly caused by sugars caramelizing.

The nutrient broth is the most critical component of the mushroom liquid culture syringe. Without a proper nutrient broth, the mushroom mycelium will not be able to survive for long. The possibility of receiving mycelium which is weak or dead on arrival is high if the vendor is using internet nutrient broth recipes that are not explicitly designed for the application of mushroom mycelium storage in a small medical-grade syringe.

Thick Spores mushroom liquid culture nutrient broth is unlike any other mushroom liquid culture seller’s nutrient broth on earth! The owner of Thick Spores explicitly designed the nutrient broth for long-term storage in a medical-grade syringe. 

No. We cannot respond if you contact us about mushroom cultivation or anything that might be illegal. We also have to cancel and prohibit your orders forever. Please never contact us about any subject matter that may be illegal.

Our customer support is availble to help you with questions related to an existing order, shipment, or our 90 day warranty.